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Dengue Fever IgG/IgM Rapid Test
Dengue is a Flavivirus that is transmitted by mosquito, principally Aedes aegypti. There are four known serotypes and serology is a useful aid in the diagnosis of dengue infections. Rapid and reliable tests for primary and secondary infections of dengue are essential for patient management. Primary Dengue infection is associated with mild to high fever, headache, muscle pain and skin rash. Immune response includes IgM antibodies produced by 5th day of symptoms and persists for 30-60 days. IgGs appear by the 14th day and persist for life. Secondary infection often result in high fever and in many cases with hemorrhagic events and circulatory failure. Secondary infection induce IgM response after 20 days of infection and IgGs rise within 1-2 days after the onset of symptoms. A reliable and sensitive rapid test that can simultaneously detect the presence of anti-dengue IgG and IgM is of great clinical utility. The Immunochromatographic format provides an excellent immune capture method for specific detection of anti-dengue IgG and IgM. The presence of high titers of IgG does not interfere with the IgM detection in the CORBANE format. A mixture of highly purified recombinant proteins corresponding to Dengue Virus E-Proteins from type 2 and 3 and covering antigenic epitopes of all 4 serotypes is conjugated to colloidal gold. The Immunochromatographic device is sensitized with goat anti-human IgG ]corresponding to a band just below the mark G ^, goat-antihuman IgM ]corresponding to a band just below the mark M ^and anti-dengue E protein monoclonal antibodies ]corresponding to the band just below the mark C ^.
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